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问题 问答题

[生物—选修1：生物技术实践](15分)临床试用抗生素前，有时需要做细菌耐药实验。实验时，首先要从病人身上获取少量样本，然后按照一定的实验步骤操作，以确定某致病菌对不同抗生素的敏感性。回答下列问题：

取该单菌落适当稀释，用______法接种于固体培养基表面，在37℃培养箱中培养24h，使其均匀生长，布满平板。

答案

参考答案：涂布

解析：【解析】：本题考查微生物的培养和应用。取该单菌落适当稀释，用涂布法接种于固体培养基表面，适宜温度培养。

单项选择题

Mr. Peter Johnson, aged twenty-three, battled for half an hour to escape from his trapped car yesterday when it landed upside down in three feet of water. Mr. Johnson took the only escape route—through the boot.
Mr. Johnson’s car had finished up in a ditch at Romney Marsin, Kent after skidding on ice and hitting a bank. "Fortunately, the water began to come in only slowly, " Mr. Johnson said. "I couldn’t force the doors because they were jammed against the walls of the ditch and dared not open the windows because I knew water would come flooding in. "
Mr. Johnson, a sweet salesman of Sitting Home, Kent, first tried to attract the attention of other motorists by sounding the horn and hammering on the roof and boot. Then he began his struggle to escape.
Later he said, "It was really a half penny that saved my life. It was the only coin I had in my pocket and I used it to unscrew the back seat to get into the boot. I hammered desperately with a hammer trying to make someone hear, but no help came. "
It took ten minutes to unscrew the seat, and a further five minutes to clear the sweet samples from the boot. Then Mr. Johnson found a wrench and began to work on the boot lock. Fifteen minutes passed by. "It was the only chance I had. Finally it gave, but as soon as I moved the boot lid, the water and mud poured in. I forced the lid down into the mud and scrambled clear as the car filled up. "
His hands and arms cut and bruised, Mr. Johnson got to Beckett Farm nearby, where he was looked after by the farmer’s wife, Mrs. Lucy Bates. Huddled in a blanket, he said, "That thirty minutes seemed like hours. " Only the tips of the car wheels were visible, police said last night. The vehicle had sunk into two feet of mud at the bottom of the ditch.

"Finally it gave" (Paragraph 5) means that ______.

A. Luckily the door was torn away in the end
B. At last the wrench went broken
C. The lock came open after all his efforts
D. The chance was lost at the last minute
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单项选择题

鉴别肝硬化和慢性缩窄性心包炎，最具有决定意义的体征是（）

A.肝硬化

B.脾大

C.腹水

D.下肢凹陷性水肿

E.肝颈静脉反流征阳性
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