问题 单项选择题 B型题

免疫组织化学PAP法的原理是()

A.将亲和素-生物素-过氧化物酶复合物中的亲和素替换为链亲和素,特异性更高

B.利用线状的葡聚糖分子与大量的AP或HRP酶结合,再将葡聚糖-酶复合物连接到第二抗体上,形成酶-葡聚糖-第二抗体复合物,灵敏度高

C.用AP取代PAP法的过氧化物酶而成,显色对比好

D.将游离酶和抗酶抗体制成稳定的酶-抗酶抗体复合物

E.通过复合物中亲和素的桥梁作用,将亲和素-生物素-过氧化物酶复合物与生物素化的抗体结合在一起,达到检测抗原的目的

答案

参考答案:D

阅读理解

Imagine climbing the three highest mountains in Britain. Now, imagine climbing all three in 24 hours. That’s what Rebecca Shedden, a British schoolgirl did and she’s only eight years old. She is one of the youngest people who have arrived on top of Snowdon, Scafell Pike and Ben Nevis, the three highest mountains in Britain.

Rebecca and her dad finished the climb, a total of about 11,000 feet (3,353 metres), in 23 hours and 15 minutes. That includes the time spent driving to each mountain: a distance of 500 miles!

Climbing isn’t new to this schoolgirl. She took her first trip up a mountain in her dad’s backpack when she was just six months old!

“One of the best parts about this climb was Scafell Pike,” said Rebecca. “I’ve never walked in the dark before, and it was fun seeing all the sheep eyes as they ran around in the dark.”

Rebecca brought along food, water, a medical kit (药箱), a hat, a map, a lamp, a camera to take photos and even a mobile phone in case (万一) something went wrong. Along the way, she and her dad had fun by making up mountain songs.

“All my friends like to stay inside playing computer games, but I like being outdoors best,” says Rebecca. Rebecca also enjoys swimming and skiing.

The young climber doesn’t plan to stay on flat land for long. She wants to climb Mount Kilimanjaro, the tallest mountain in Africa. “And I would like to climb Mount Qomolangma one day, too,” she said.

小题1:What is the distance up and down all these three highest mountains in Britain?

A.500 miles

B.24 hours’ walk

C.11,000 feet

D.23 hours and 15 minutes’ drive小题2:Why did Rebecca like climbing Scafell Pike best of the three trips?

A.Because the Scafell Pike was the darkest part.

B.Because it is the highest mountain in Britain.

C.Because she likes being outdoors best.

D.Because she saw all the sheep eyes in the dark.小题3:Rebecca likes climbing probably because ______.

A.she doesn’t get along well with her friends

B.her dad has a great influence on her

C.she likes making up mountain songs

D.she wants to climb Mount Kilimanjaro

多项选择题