某患者有过不涪性交史，1个月后外生殖器出现丘疹状硬结而来就诊，检查发现其硬结呈圆形，基底清晰，边缘隆起，质硬，有无痛性溃疡。初步怀疑为梅毒螺旋体感染。
如果取该患者硬结的分泌物做涂片检查，则下列哪种检查方法为宜（）
A.革兰染色后油镜检查
B.不染色直接油镜下检查
C.暗视野显微镜检查
D.相差显微镜检查
E.电镜检查
参考答案：C
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| Love in a Box When I was a little girl, I found love in a box all because of a class assignment. On a Friday night I 1 at the dinner table, "My teacher said we have to bring a box, a special box, for our Valentines (情人节) on Monday." Mother said, "We'll see", and she continued eating. What did "We'll see" mean?I had to have that box 2 my second grade Valentine's Day would be a disaster. Maybe they didn't love me enough to help me with my 3 . All Saturday I waited 4 and with Sunday arriving, my concern increased, but I 5 an enquiry about the box might 6 anger or loud voice, for in my house children only asked once. More than that 7 trouble. Late Sunday afternoon, my father called me into the kitchen. The table was covered with colorful 8 of different kinds. A(n) 9 shoebox rested on top of it. 10 flooded through me when Daddy said, "Let's get started 11 your project." In the next hour my father 12 the shoebox into an impressive valentine box. Colorful paper covered the ugly cardboard with red hearts 13 to what I considered all the right places .He sang while he worked. When he finished, he was so delighted that a 14 smile spread over his face. "What do you think of that?" he asked. I answered him with a hug. But inside, 15 danced all the way to my heart. It was the first time that my father devoted so much 16 to me, for his wo  rld consisted of work only.The holiday party arrived, and my classmates put cards, and presents into the valentine boxes. Laughter filled our classroom until dismissal time 17 . On the way home, I held out my valentine box for the world to 18 .The love that filled it meant more to me than all inside. The valentine box became a symbol of his love that 19 through decades of other Valentine's Days. He gave me other gifts through the years, but none 20 compared with the love I felt within the limits of the old, empty shoebox. | ||||
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