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问题 单项选择题

能测定蛋白质分子量的电泳技术是

A．醋酸纤维薄膜电泳

B．琼脂糖凝胶电泳

C．火箭免疫电泳

D．免疫固定电泳

E．SDS-PAGE

答案

参考答案：E

解析： 在聚丙烯酰胺系统中加入阴离子去污剂十二烷基磺酸钠(SDS)，大多数蛋白质能与SDS按一定比例结合，蛋白质结合SDS复合物都带上相同密度的负电荷，它的量大大超过了蛋白质分子原有的电荷量，因而消除了蛋白质原有的电荷差别，使蛋白质，分子电泳的迁移率主要取决于本身的分子量，而与蛋白质所带的电荷无关，在一定条件下，蛋白质的分子量的对数与电泳迁移率间呈负相关。

多项选择题 案例分析题

患者，女性，62岁，因上腹部不适、疼痛3天，发热38.2℃，以上消化道穿孔，腹膜炎入院。拟急诊行剖腹探查术。患者既往糖尿病史20年，口服降糖药控制血糖。高血压病史3年，口服降压药，血压控制满意。入院后血糖为13.3mmol／L。

术前应做什么检查（）。

A.24小时动态血压监测

B.电解质

C.血液生化

D.尿常规查尿糖和尿酮体

E.动脉血气
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填空题

Stress is our response to threatening or (1) events. We may experience different levels of stress depending on the (2) , meaning the events or circumstances that cause us to feel stress. Stressors can be classified into (3) main categories: (4) events, personal stressors, and (5) stressors. Cataclysmic events are major events that cause stress (6) , immediately, for a great many people at once. Personal stressors are major life events that create stress, including (7) life events. Background stressors are (8) hassles, or minor irritations, or (9) background stressors. Repeated exposure to stressors has both psychological and (10) consequences. The long- term consequence is that body tissues such as the heart and blood vessels can begin to (11) . The (12) system functions less effectively, and (13) our ability to fight off illnesses.
The General Adaptation (14) explains the sequence of physiological reactions to stress. There are three phases to G. A. S. The first is the " alarm and (15) " phase: we respond with alarm, and take (16) to remove the stressor. The second phase is the (17) stage: we fight against the stressor or try to cope with the stressor. This can lead to the third phase. (18) . The exhaustion phase may be an (19) way of trying to avoid the stressors. The body may be telling us that we need to do whatever is (20) to remove the stressor.

Stress is our response to threatening or (1) events. We may experience different levels of stress depending on the (2) , meaning the events or circumstances that cause us to feel stress. Stressors can be classified into (3) main categories: (4) events, personal stressors, and (5) stressors. Cataclysmic events are major events that cause stress (6) , immediately, for a great many people at once. Personal stressors are major life events that create stress, including (7) life events. Background stressors are (8) hassles, or minor irritations, or (9) background stressors. Repeated exposure to stressors has both psychological and (10) consequences. The long- term consequence is that body tissues such as the heart and blood vessels can begin to (11) . The (12) system functions less effectively, and (13) our ability to fight off illnesses.
The General Adaptation (14) explains the sequence of physiological reactions to stress. There are three phases to G. A. S. The first is the " alarm and (15) " phase: we respond with alarm, and take (16) to remove the stressor. The second phase is the (17) stage: we fight against the stressor or try to cope with the stressor. This can lead to the third phase. (18) . The exhaustion phase may be an (19) way of trying to avoid the stressors. The body may be telling us that we need to do whatever is (20) to remove the stressor.
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