高压蒸汽灭菌器灭菌不彻底的指标是（）

A.灭菌后取出包裹在被灭菌物体中的温度计，可见读数超过121℃

B.温度记录仪显示锅内温度已达121℃，且维持20min以上

C.灭菌后将放在待灭菌物中浸有嗜热脂肪杆菌芽胞的菌片放入溴甲酚紫葡萄糖胰蛋白胨培养液中，置56℃培养48h，培养液变为黄色

D.将硫磺、苯甲酸粉或结晶放入灭菌器内，灭菌完毕冷却后放入的试剂凝固成团块

E.灭菌后将放在待灭菌物中浸有嗜热脂肪杆菌芽胞的菌片放入溴甲酚紫葡萄胰蛋白胨培养液中，置56℃培养48h，培养液仍为紫色

将下列句子改为含有宾语从句的复合句。

1. Does Mr Brown enjoy living in China? Could you tell me?

    Could you tell me _____  Mr Brown  _____ living in China?

2. "No news is good news. "he said.  He said _____ no news _____ good news.

3. Who invented TV set?  I don't know. I don't know ______  _____ TV set.

4. " Does the girl need any help?" he asked me. He asked me _____ the girl ____ some help.