简述固体培养基的配制和灭菌方法
参考答案：
实验室配制固体培养基的操作程序是照配方称取药品，将药品溶解在一定量的水中后加热煮沸，将琼脂按量称取后用水浸湿，将浸湿的琼脂加入煮沸的营养液中，待琼脂全部融化后调节pH值，补充损失的水分，分装培养基入不同的容器中。
使用手提式灭菌锅进行灭菌的操作程序是：锅内加水，灭菌物体装锅，对称上紧密封螺帽将锅密封上，加热升温，排尽锅内冷空气，升温至灭菌工艺条件（一般为98kpA.，在工艺条件下保压计时（一般为30分钟），到时间后切断热源，降温，出锅。
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| Have you ever planted a few trees on Tree Planting Day? Each year, millions of people, both old and young, 1 a lot of trees. But this may not be the best way to make your home a 2 place. Last week we visited a park in Hebei. We were 3 to find most of the trees had been burned. The workers told us that the trees 4 soon after they were planted because they weren't watered well enough. 5 the workers burned them and cleaned the place for 6 trees to be planted this year. 7 a card found in one of the dead trees, a student wrote: "I hope this tree will grow up with me to 8 the backbone(栋梁) of our country." The workers said that the survival of the trees was really more important than 9 trees were planted. Some people in Beijing now have a new idea that they can donate some money and let professionals(专业人士) plant and 10 the trees. | |||
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