问题 填空题

已知蛋白质混合液中硫酸铵浓度的不同可以使不同种类的蛋白质析出(或沉淀),随着硫酸铵浓度增加,混合液中析出的蛋白质种类和问题增加。下表是某蛋白质混合液中的不同蛋白质从开始析出到完全析出所需要的蛋白质混合液中的硫酸铵浓度范围。

请据表回答:

(1)若只完全析出甲蛋白,混合液中最合适的硫酸铵浓度应为___________。

(2)向该蛋白质混合液中加入硫酸铵溶液(或硫酸铵),使混合液中的硫酸铵浓度达到30%,会析出若干种蛋白质,它们分别是______________。

(3)通过改变混合液中的硫酸铵浓度_________(能、不能)从混合液中得到所有的、不含有其他蛋白质的乙蛋白,原因是___________。

(4)简要写出从该蛋白质混合液中分离出全部丁蛋白的实验设计思路。

(5)如果蛋白质析出物中还含有一定量的硫酸铵,可用半透膜除去析出物中的硫酸铵。用半透膜能除去析出物中硫酸铵的原理是_________________。

答案

(1)20%

(2)甲蛋白、乙蛋白、丙蛋白

(3)不能   乙蛋白和丙蛋白析出所需的硫酸铵浓度范围有重叠

(4)向该蛋白质混合液中加入硫酸铵溶液(或硫酸铵),使其浓度达到35%(或35%≤硫酸铵浓度<38%)范围内任意一个浓度;分离析出物与溶液,保留溶液,取保留溶液,再加入硫酸铵溶液(或硫酸铵),使硫酸铵在溶液中的浓度达到40%(或40%以上),分离析出物与溶液,析出物即为丁蛋白。

(5)半透膜是一种选择性透过膜,只允许小分子的硫酸铵通过,不允许大分子蛋白质通过。

阅读理解

Do you have any foreign friends? Do you know their characteristics(特征)?

The Germans are very quiet and they always keep calm. They don’t like to speak more words. They look very serious. They like different kinds of amusements. The Germans are very hard—working. They like tidiness, especially the women, who always keep their home clean.

In some ways, the Englishmen look the same as the Germans. They are very quiet and never talk too much with the strangers. They are really polite, so we often hear they say “Thank you.” Or “Sorry.” The gentlemen are also the Englishmen.

The French’s holidays are very long. They like traveling and usually spend their long time staying in other countries. The Frenchmen are more outgoing than the Germans. It is very easy to make friends with them.

Compared to the Frenchmen, the Americans are more outgoing. And they are even opener than all of the Europeans. They don’t like to depend on others. So it is very common that the students do part-time jobs in their free time. And in Americans’ eyes, success is an important part in their life.

小题1:According to the passage, ______are very quiet.

A.only the Germans

B.only the Englishmen

C.only the Frenchmen

D.both the Germans and the Englishmen小题2: What does the underlined word “tidiness” mean in Chinese?

A.整洁

B.安静

C.时尚

D.随意小题3:In the writer’s eyes, the gentlemen are______.

A.the Germans

B.the Frenchmen

C.the Englishmen

D.the Americans小题4:What can we learn about the Frenchmen from the passage?

A.They are more outgoing than the Americans.

B.It is very easy to make friends with them.

C.They like traveling in their own country.

D.They look serious and are always hard-working.小题5:Which is the best title of the passage?

A.Foreigners’hobbies.

B.Who are more outgoing?

C.Characteristics of the foreigners.

D.Success is an important part in Americans’life.

单项选择题