问题 单项选择题

现在医学科学工作者通过获得大量特异 DNA,结合适当的分析技术即可鉴定基因缺陷。当前临床或研究室获得大量特异 DNA片段最流行的方法是( )。

A.化学合成

B.DNA合成仪合成

C.从外周血细胞大量制备

D.基因克隆

E.聚合酶链反应

答案

参考答案:E

解析: 聚合酶链反应技术(PCR)是利用人工合成的核苷酸序列作为“引物”,在耐热DNA聚合酶的作用下,通过变化反应温度,扩增目的基因,用于检测体液,组织中相应核酸的存在,在扩增循环中DNA片段上百万倍增加是很特异和非常灵敏的方法。

完形填空

Do you know who invented tea? It is said that the emperor Shen Nong  discovered   tea when he was boiling drinking water 21 an open fire. Some leaves from a nearby tree fell into the water and the leaves were in the water for  22 . The emperor noticed that the leaves in the water produced a  23   smell. Later he decided 24  the hot water with the leaves. It was quite delicious. And  25 , one of the worl’s favorite drinks was invented.
26 is the home of tea. And tea has more than 4,000 years history. 27  the three drinks—tea, coffee and cocoa, tea is drunk by  28  people in the world. Tea  29 China began to be known by the world over a thousand years ago and has been an important Chinese export (出口商品) 30  .
Tea leaves  31  mainly in the south of the Yangtze River, 32 the warm climate (气候)there. Longjing, Wulong, Pu’er and Tieguanyin are all  33   kinds of tea.
Over the past centuries, Chinese people  34   their tea culture(文化), including tea planting, tea-leaf picking, tea making and so on. We know tea is  35   a popular topic(主题) in dances, songs, poems and novels.
小题1:
A.in B.byC.overD.to
小题2:
A.sometimesB.some timeC.some timesD.some days
小题3:
A.pleasantB.pleasedC.badD.terrible
小题4:
A.not to tasteB.to tasteC.tastingD.taste
小题5:
A.by the wayB.on the wayC.on its wayD.in this way
小题6:
A.JapanB.ChinaC.AmericaD.England
小题7:
A.InB.ForC.OfD.By
小题8:
A.the number ofB.a number of
C.a large number ofD.the largest number of
小题9:
A.toB.fromC.inD.off
小题10:
A.At that timeB.from now onC.since thenD.since now
小题11:
A.produceB.are producedC.makeD.are made
小题12:
A.becauuse of B.becauseC.asD.for
小题13:
A.interestingB.importantC.famousD.expensive
小题14:
A.had developedB.have developedC.developedD.are developing
小题15:
A.tooB.as wellC.eitherD.also
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