问题 问答题 简答题

典型的DNA重组实验通常包括哪些步骤?

答案

参考答案:

a、提取供体生物的目的基因(或称外源基因),酶接连接到另一DNA分子上(克隆载体),形成一个新的重组DNA分子。

b、将这个重组DNA分子转入受体细胞并在受体细胞中复制保存,这个过程称为转化。

c、对那些吸收了重组DNA的受体细胞进行筛选和鉴定。

d、对含有重组DNA的细胞进行大量培养,检测外援基因是否表达。

阅读理解

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