某患者剧烈头痛，喷射状呕吐，皮肤出血性淤斑。查脑膜刺激征（+）。分离培养可能的病原菌应注意的事项中不包括（）
A.可采集脑脊液
B.可采集血液或出血淤斑
C.标本要立即送检，并注意保湿保暖
D.需补充5%～10%CO2气体
E.标本直接接种在巧克力血琼脂平板上
参考答案：E
解析：
患者症状与流行性脑脊髓膜炎相符。分离脑膜炎球菌应注意的事项有：
①可采集脑脊液、血液、出血瘀斑作标本；
②脑膜炎球菌产生自溶酶，对干燥、热力敏感，在室温下3h即死亡，故标本要立即送检、注意保温。培养基要预温；
③血液或脑脊液中菌量可能少，为提高分离率，应先接种至血清肉汤培养基增菌后，再在巧克力血平板上行划线分离；
④初次分离需补充CO2。
| Many researchers have found greater dependence and obedience in very young girls, greater autonomy and activity in boys. When a barrier is set up to separate children from their mothers, boys try to knock it down; girls cry helplessly. There is little doubt that mother’s encouragement or discouragement—of such behavior plays a major role in determining adult personality. For example, a mother often stimulates male autonomy by throwing a toy far away from her young son, thus silently suggesting to him that he leaves her to get it. Animal studies suggest that there may be a biological factor in material behavior; mothers of some monkeys punish their male babies earlier and more often than their female offspring; they also touch their female babies more often and act more protectively toward them. As for the controversial question of female "passivity", psychologist Helen Deutsh believes that the concept has been misunderstood. "There is no contradiction between being feminine and working. The self can be active in both men and women," she says. It is only in love and sex that passivity is particularly appropriate for women. As she sees it, passivity is no more than a king of openness and warmth; it does not mean "inactivity of emptiness". Another controversy ranges over the effect of hormones. Aggressive women; who discount hormonal influence, disagree violently with scientific researchers, who almost unanimously agree that hormones help determine how people feel and act. So far, there have been few studies of male hormones, but scientists think they may eventually discover hormonal cycles in men that produce cyclic changes in mood and behavior. As for females, studies have indicated that 49 percent of female medical and surgical admissions, most psychiatric hospital admission and 62 percent of violent crimes among women prisoners are related with hormonal cycles in women. |
Which of the following is NOT true of the study of hormones
A．There are disputes to the effect of hormones.
B．Most women know that hormones have much to do with people’s behavior.
C．Nearly all researchers believe that hormones directly link to people’s action and feeling.
D．Aggressive women don’t believe the effect of hormones so much as researchers do.