情景对话

根据对话情景和内容，从对话后所给的选项中选出能填入每一空白处的最佳选项。选项

中有两个为多余选项。

Tom: What did you think of the lecture?

Mary:  1   

Tom: Did you really?

Mary:   2  

Tom: Certainly not .   3  

Mary: So you think you know more than he does.

Tom: Well, take coal for instance.

Mary:   4   

Tom: Coal won't become important again.

Mary: Why not?

Tom: It's too dirty. They won't be able to find people to work down coal mines in the future.

Mary:    5  

Tom: Nonsense. The only sort of power they will use in the future is atomic power.

A. What about it?

B. I thought he talked a lot of rubbish.

C. I thought it was very interesting.

D. What is it?

E. Yes, didn't you?

F. They'll invent new kinds of machinery.

G. No, did you?

生物分子间的特异性结合的性质广泛用于生命科学研究。以下实例为体外处理“蛋白质-DNA复合体”获得DNA片段信息的过程图。据图回答：

(1)过程①酶作用的部位是                键，此过程只发生在非结合区DNA，过程②酶作用的部位是                键。

(2)①、②两过程利用了酶的                 特性。

(3)若将得到的DNA片段用于构建重组质粒，需要过程③的测序结果与           酶的识别序列进行对比，已确定选用何种酶。

(4)如果复合体中的蛋白质为RNA酶聚合，则其识别、结合DNA序列位基因的                  。

(5)以下研究利用了生物分子间的特异性结合的有              (多选)

A．分离得到核糖体，用蛋白酶酶解后提rRNA

B．用无水乙醇处理菠菜叶片，提取叶绿体基粒膜上的光合色素

C．通过分子杂交手段，用荧光物质标记的目的基因进行染色体定位

D．将抑制成熟基因导入番茄，其mRNA与催化成熟酶基因的mRNA互补结合，终止后者翻译，延迟果实成熟。