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科学家从某些能无限增殖细胞的细胞质中分离出无限增殖调控基因(prG)，该基因能激发许多动物细胞分裂，这为单克隆抗体的制备提供了更为广阔的前景。请回答下列问题：

(1)利用细胞融合技术制备单克隆抗体的过程中，促进动物细胞融合常用的诱导剂是____。

(2)利用细胞融合技术制备单克隆抗体时有两次筛选，第1次筛选的目的是_______________，第2次筛选的目的是__________________。

(3)有人提出，可以直接通过基因工程导入该调控基因来制备单克隆抗体，其思路如图所示。请回答下列问题：

①．酶A是指_________，酶B是指_________。

②．对已导入重组质粒的Ⅱ进行“检测”的目的是__________；题目中Ⅱ所指的细胞是_________，Ⅲ所指的细胞具有的特点是________________。

(4)也有人提出用细胞核移植技术构建重组细胞来生产单克隆抗体，试用相关的图示及文字表示该过程：

答案

(1)灭活的病毒

(2)筛选出杂交瘤细胞   筛选出能产生所需抗体的杂交瘤细胞

(3)①．限制性(核酸)内切酶   DNA连接酶   ②．检测目的基因在受体细胞中是否进行了表达(或检测受体细胞是否显示出目的基因控制的性状)   效应B细胞   既能无限增殖又能产生特异性抗体

(4)

单项选择题

患者女，22岁。经期提前半年余，每次提前10天左右，月经量多，色深红，质黏稠，伴面红口干，心胸烦热，小便短赤，大便干燥，舌红，苔黄，脉数。治疗除主穴外，还应加用（）。

A.太溪、肾俞

B.足三里、三阴交

C.太冲、行间

D.脾俞、足三里

E.气海、关元
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填空题

World Water Shortage


A new study warns that about thirty percent of the world’s people may not have enough water by the year 2025.
A private American organization called (1) Action International did the new study. It says (2) than three-hundred-thirty-five-million people (3) enough water now. The people live in twenty-eight (4) . Most of the countries are in Africa or the (5) East.
P-A-I researcher Robert Engelman says (6) the year 2025, about three-thousand-million people (7) lack water. At least 18 more countries are (8) to have severe water problems. The demand (9) water keeps increasing. Yet the amount of water on Earth (10) the same.
Mr. Engelman says the population in countries that lack water is (11) faster than in other parts of the world. He says (12) growth in these countries will continue to (13) .
The report says lack of water in the future may (14) in several problems. It may increase health (15) . Lack of water often means drinking (16) not safe. Mr. Engelman says there are problems (17) over the world because of diseases, such as cholera, (18) are carried in water. Lack of water may also result (19) more international conflict. Countries may have to (20) for water in the future. Some countries now get sixty percent of their fresh water from other countries. This is true of Egypt, the Netherlands, Cambodia, Syria, Sudan, and Iraq. And the report says lack of water would affect the ability of developing to improve their economies. This is because new industries often need a large amount of water when they are beginning.
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