某研究小组为测定药物对体外培养细胞的毒性，准备对某种动物的肝_爱下问搜题

搜题请搜索小程序“爱下问搜题”

问题选择题

某研究小组为测定药物对体外培养细胞的毒性，准备对某种动物的肝肿瘤细胞(甲)和正常肝细胞(乙)进行动物细胞培养。下列有关说法正确的是[ ]

A．在利用两种肝组织块制备肝细胞悬液时，可用胃蛋白酶处理

B．细胞培养应在CO₂培养箱中进行，CO₂的作用是作为细胞代谢原料

C．取单个肝肿瘤细胞进行培养，获得细胞群的方法不属于克隆培养法

D．乙细胞在原代培养过程中，最终会出现停止增殖的现象

答案

答案：D

单项选择题案例分析题

患者女，15岁，于入院前5d突发上呼吸道感染，体温最高达39℃。后突然出现晕厥，伴双眼上翻、肢体抽搐，无口吐白沫及尿、粪失禁，数秒钟后恢复。后再发1次，症状如前。遂至当地医院就诊。入院查体示左肺呼吸音清，右下肺呼吸音低。

此时，考虑的疾病是（）（提示：患者入院前出现晕厥，住院期间出现腹痛。腹部B型超声示少量积液；肝右叶高回声，考虑良性病变；慢性肾功能不全图像。ALT3632U/L，AST1896U/L；尿素氮36.2mol/L，肌酐4464mol/L。）

A.腹型癫痫

B.心源性肝、肾损伤

C.急性肾小球肾炎

D.病毒性肝炎

E.急性胰腺炎

F.急性阑尾炎

完形填空

While waiting to pick up a friend at the airport in Portland, Oregon, I noticed a man coming toward me carrying two light bags. He stopped right next to me to       his family.
First he moved to his youngest son as he laid down his bags. They gave each other a long, loving hug.       they separated, I heard the father say, “It’s so good to see you, son. I missed you so much!” His son smiled somewhat shyly and replied softly, “Me, too, Dad!”
Then the man stood up, gazed in the       of his oldest son and said, “You’re already quite the young man. I love you very much, Zach!” They too hugged a most loving, tender hug.
After several       , he turned to his wife and declared, “I’ve saved the best for last!” and      to give his wife the longest, most passionate kiss I       remember seeing.
For an instant they       me of newlyweds(新婚夫妇), but I knew by the age of their kids that they couldn’t possibly be. I puzzled about it for a moment and gathered my courage to      ask, “Wow! How long have you been married?”
“Almost twelve years.” he replied,       breaking his gaze from his lovely wife’s face. “Well then, how long have you been away?” I asked. The man      turned and looked at me,. “Two whole days”
Two days? I was      . By the intensity of the greeting, I had       he’d been gone for at least several weeks — if not months. I know my expression      me. Hoping to stop our conversation politely and continue with my       for my friend, I said almost immediately, “I hope my        is still that passionate after twelve years!”
The man suddenly       smiling. He looked me straight in the eye, and with power that burned right into my soul, he told me something that left me a        person. He told me, “Don’t    , friend …decide!” Then with wonderful smile, he and his family turned and left together.
I was still watching that special family walk just out of sight when my friend        to me and asked, “What are you looking at?” Without      , and with a curious sense of certainty, I replied, “ My future!”

D．deliberately

D．discouraged