问题 实验题

进行植物体细胞杂交,首先需要除去细胞壁。现提供以下材料和用具:紫色洋葱鳞片叶、适宜浓度的盐酸、适宜浓度的蔗糖溶液(洋葱细胞在此溶液中既不吸水也不失水)、纤维素酶、果胶酶、蒸馏水和镊子、显微镜、载玻片、盖玻片等。请你从中选取合适的材料配制成合理的细胞壁分解液,并设计实验验证该分解液能除去细胞壁。

(1)配制细胞壁分解液选取______________,这样选取的理由是_________________。

(2)实验步骤:

第一步:_____________________________制得实验组装片;

第二步:_____________________________制得对照组装片;

第三步:在显微镜下观察比较实验组和对照组装片。

(3)实验结果的预测:实验组装片的洋葱细胞呈___________________,表明细胞壁已经被水解;对照组装片的洋葱细胞呈_____________________,表明细胞壁未被分解。

答案

(1)适宜浓度的蔗糖溶液加入适量的纤维素酶和果胶酶; 纤维素酶和果胶酶能高效、专一地水解细胞壁,适宜浓度的蔗糖溶液可以使原生质体不会因过度吸水而涨破。

(2)第一步:滴适量的细胞壁分解液在载玻片中央,用镊子撕取一小块紫色洋葱鳞片叶表皮浸泡在其中,一段时间后,盖上盖玻片;第二步:用适宜浓度的蔗糖溶液替代细胞壁分解液,其他操作与制作实验组装片相同;

(3)实验组装片的洋葱细胞呈分散的紫 * * 形;对照组装片的洋葱细胞呈相互连接的紫色多边形。

完形填空
完形填空。
     Our school has a library. There are many  1  in it. All the students in our school can borrow books  2  
the library, but you can't  3  them for more than two weeks. Everyone must give back the library books  4 .
One afternoon, Miss Yang was working at her desk in the library when Han Mei  5  ." Excuse me, Miss Yang,"
She said." I'm very sorry. I borrowed a book ten days ago. But I can't  6  it. I think I've lost it.""Oh dear!"
said Miss Yang,"What was the name of the book?"" It was 'Harry Potter'.""What a pity! I'm  7  that if you've
lost it, you must pay for (偿还) it," said Miss Yang. Just then Lucy came into the library." Excuse me, Miss
Yang. I've just found this book. I like this book very much, but I think it's the  8 , so I come to  9  ." "Oh,
good girl!" Miss Yang got the book and said."It's 'Harry Potter'. Come on, Meimei! Is it the book you've
lost?" "Yes," Han meimei said." Thank you, Miss Yang!""Don't thank me, we  10  thank Lucy!" said Miss
Yang.
( )1. A. kind of books
( )2. A. from         
( )3. A. keep         
( )4. A. in the time  
( )5. A. comes        
( )6. A. find         
( )7. A. sadly        
( )8. A. library      
( )9. A. given it back
( )10. A. and         
B. kinds of books
B. at           
B. borrow       
B. at times     
B. came in      
B. looks        
B. sorry        
B. library's    
B. give it back 
B. both         
C. kinds of book
C. out         
C. had         
C. on time     
C. will come   
C. looking     
C. glad        
C. Lily's      
C. gave back   
C. or          
D. kind of book
D. to          
D. has         
D. some time   
D. was come    
D. found                             
D. happy       
D. Lucy's      
D. gave it back
D. either      
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