问题 读图填空题

下图是从酵母菌获取某植物需要的某种酶基因的流程,结合所学知识及相关信息回答下列问题:

(1)图中cDNA文库_______(填“大于”“等于”或“小于”)基因组文库。

(2)①过程提取的DNA需要_________的切割,B过程是__________。

(3)为在短时间内大量获得目的基因,可用________扩增的方法,其原理是___________。

(4)目的基因获取之后,需要进行________,其组成必须有__________以及标记基因等。此步骤是基因工程的核心。

(5)将该目的基因导入某双子叶植物细胞,常采用的方法是___________,其能否在此植物体内稳定遗传的关键是_________可以用_________技术进行检测。

(6)如果要想使该酶活性更强或具有更强的耐受性,需要对现有蛋白质进行改造,这要通过基因工程延伸——蛋白质工程。首先要设计预期的_______,再推测应有的氨基酸序列,找到相对应的_________。

(7)除植物基因工程硕果累累之外,在动物基因工程、基因工程药物和基因治疗等方面也取得了显著成果,请列举出至少两方面的应用:__________________。

答案

(1)小于

(2)限制酶   反转录

(3)PCR技术   DNA复制

(4)基因表达载体的构建   启动子、终止子、目的基因(复制原点可不答)

(5)农杆菌转化法   目的基因是否整合到植物细胞的染色体上   DNA分子杂交

(6)蛋白质结构   脱氧核苷酸序列

(7)乳腺生物反应器,体外基因治疗复合型免疫缺陷综合征(其他合理均给分)

完形填空
Twist was the name of an old champion racing horse. He was past thirty years old, but the heart of the true  36  still beat with the great spirit. However, I knew little about the   37  that the old horse’s spirit would have on my daughter.
My daughter Stacy had ever had a   38  experience with a runaway horse. She was just eight at the time, and a terrifying   39  accompanied the disaster. Although she broke no bones, her   40  , love for horses and the desire to learn to ride were   41 . No matter how my wife and I tried, we had no   42  in curing the damage caused that day. But when she was thirteen, a turning point came. One day as I saw Stacy look into the eye of the   43  fellow in the stall(牲口棚), I knew this was going to be the beginning of a   44  relationship.
Fortunately, Stacy’s accident had not weakened her love for   45  in general, and this small opening was all that Twist needed to create an unusual bond(纽带). In the following weeks, Stacy began to express daily   46  in coming to the stall with me. She   47  spent time with him, feeding, brushing and combing him, all the  48  talking to him about her life.
Then one day   49  I was readying one horse for a ride, I noticed the old guy’s   50 expression and desire to be included, too. So I asked Stacy if she wanted to take Twist out for a ride. In   51 , Stacy looked once again into the old man’s eye. That moment, their two spirits   52  and completed the bond that had been forming over a couple of months. Continuing to look deep into his eyes, Stacy didn’t   53  . She only nodded yes. Moments later, I saw them riding   54  together, Stacy winning renewed confidence and desire, and Twist winning his medal of a child’s  55 .
小题1:
A.ownerB.winnerC.jumperD.member
小题2:
A.wishB.ideaC.effectD.ability
小题3:
A.newB.badC.richD.strange
小题4:
A.fallB.taskC.shakeD.attack
小题5:
A.prideB.skillC.luckD.confidence
小题6:
A.ignoredB.reformedC.destroyedD.overlooked
小题7:
A.aimB.needC.surpriseD.success
小题8:
A.poorB.oldC.brave D.strong
小题9:
A.seriousB.similarC.specialD.formal
小题10:
A.animalsB.parentsC.friendsD.fields
小题11:
A.thanksB.interestC.opinionsD.sympathy
小题12:
A.endlessly B.roughlyC.unwillingly D.actively
小题13:
A.bestB.wayC.sameD.time
小题14:
A.asB.soC.althoughD.because
小题15:
A.angryB.sadC.eagerD.anxious
小题16:
A.briefB.responseC.returnD.addition
小题17:
A.metB.roseC.foughtD.recovered
小题18:
A.actB.thinkC.speakD.smile
小题19:
A.onB.backC.downD.off
小题20:
A.heartB.spiritC.agreementD.dependence
单项选择题