问题 读图填空题

(选做题)根据基因工程的有关知识,回答下列问题: 

(1)限制性内切酶切割DNA分子后产生的片段,其末端类型有_______和______。

(2)质粒运载体用EcoRⅠ切割后产生的片段如下:

为使运载体与目的基因相连,含有目的基因的DNA除可用EcoRⅠ切割外,还可用另一种限制性内切酶切割,该酶必须具有的特点是_________。

(3)按其来源不同,基因工程中所使用的DNA连接酶有两类,即________DNA连接酶和_____DNA连接酶。

(4)反转录作用的模板是________,产物是__________。若要在体外获得大量反转录产物,常采用__________技术。

(5)基因工程中除质粒外,__________和__________也可作为运载体。

(6)若用重组质粒转化大肠杆菌,一般情况下,不能直接用未处理的大肠杆菌作为受体细胞,原因是_____________。

答案

(1)平末端       粘性末端

(2)切割产生的DNA片段末端与EcoRⅠ切割产生的相同

(3)大肠杆菌      T4

(4)mRNA       cDNA   PCR

(5)噬菌体      动植物病毒

(6)未处理的大肠杆菌吸收质粒(外源DNA)的能力极弱

单项选择题
单项选择题

Turkey’s Bodrum peninsula is different. The tourist boom in this part of the world (1) turned some small villages into resorts yet left neighbouring beaches undisturbed, making it quite (2) southern France or the Spanish Coasts where few stretches of coastline are undevelopeD.
The (3) for this happy set of circumstances is simple. For thousands of years, travel here (4) easier by boat than by lanD.So when mass tourism arrived in the (5) 1980s, there was no coast road for ribbon development to follow. So the peninsula, just (6) hour from Bodrum airport, has not become one long littoral of resort.
The building (7) new hotels has mainly been confined to places easily reached by then relatively (8) roads. Such ease of access has made Gumbet, near Bodrum, a busy resort, while the little fishing village of Gumusluk, 12 miles further west and only recently reachable by (9) , remains tranquil and undisturbeD.
It’s worth thinking carefully about location when planning a family (10) on the peninsulA.Choose a place that is centrally located, preferably out of earshot (11) Bodrum town’s "lively"—which means nosy—nightlife, and you can then use (12) area’s comfortably small scale to your advantage. Today’s new roads mean most places can (13) reached in under an hour by taxi or the ubiquitous dolmus-minibus.
The (14) of facilities at the Tamarisk Beach Hotel near the small village of Ortakent makes (15) a good base. The family-run hotel—rooms and suites are in two-storey buildings (16) by palm trees and flowers in terracotta pots—sits above its own sandy (17) beach, shaded by tamarisk trees and sheltered by nearby islands.
The hotel is (18) child-friendly, too. Children, from infants up to young teenagers, can take part in a (19) of games and activities that include tuition in windsurfing, dinghy (20) catamaran sailing.

When mentioning media, the Secretary-General meant that media play the role of______.

A.investing funds in tourism development

B.making advertisements for tourism industry

C.spreading the message of Tourism Enriches campaign

D.stimulating economic activities